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Extra info for AHFS Drug Information 2004
Helicase (rep protein) continuously unwinds duplex DNA at the replication fork during synthesis d. Nucleoside monophosphates are added in a 5′ to 3′ direction along the growing DNA chain e. qxd 11/14/01 10:36 AM Page 17 DNA Structure, Replication, and Repair 17 23. Which of the following statements describing restriction endonucleases is true? a. b. c. d. e. They always yield overhanging single-stranded ends They recognize methylated DNA sequences They recognize triplet repeats They cleave both strands in duplex DNA They always yield blunt ends 24.
A. Isolation of DNA from red blood cells followed by polymerase chain reaction (PCR) amplification and restriction enzyme digestion b. Isolation of DNA from blood leukocytes followed by Southern blot analysis to detect globin gene exon sizes c. Isolation of DNA from blood leukocytes followed by DNA sequencing of globin gene introns d. Isolation of DNA from blood leukocytes followed by polymerase chain reaction (PCR) amplification and allele-specific oligonucleotide (ASO) hybridization e. Western blot analysis of red blood cell extracts 37 Copyright 2002 The McGraw-Hill Companies.
Two-base insertions would change the reading frame of the genetic code (frame-shift mutation) and produce a nonsense peptide after the point of insertion. Three-base deletions could also cause frame shifts or, if one codon were removed, delete one amino acid. Nondisjunction involves abnormal segregation of chromosomes at meiosis or mitosis, and would produce nonviable individuals or somatic cells with additional or missing copies of chromosome 11 and its β-globin locus. 16. The answer is e. (Murray, pp 412–434.