By Corinne Whitby, Torben Lund Skovhus
Applied Microbiology and Molecular Biology in Oil box platforms addresses the key difficulties microbes reason in oil fields, (e.g. biocorrosion and souring) and the way helpful microbial actions can be exploited (e.g. MEOR and biofuels). The publication describes theoretical and useful ways to express Molecular Microbiological equipment (MMM), and is written through best specialists within the box from either academia and undefined. The e-book describes how MMM should be utilized to faciliate higher administration of oil reservoirs and downstream techniques. The publication is cutting edge in that it utilises genuine commercial case reviews which provides important technical and clinical info to researchers, engineers and microbiologists operating with oil, fuel and petroleum systems.
Read or Download Applied Microbiology and Molecular Biology in Oilfield Systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009 PDF
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Additional info for Applied Microbiology and Molecular Biology in Oilfield Systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009
A) Artist impression of the double helix structure of DNA (simplified). (b) Schematic representation of the DNA base pairing and hydrogen bridges, with Adenine, Thymine, Guanine, Cytosine. Clearly shown is that the AT base pair holds only two H-bridges, while the GC base pair holds three. The connecting molecule bonds and structures are commonly known as the DNA backbone and consist of ribose and phosphate groups. The extra hydrogen bridge in the GC base pair is the key to the separation of PCR products in the DGGE technique.
After the DGGE analysis the gels were stained using a dye that binds to genomic material and photographed revealing fingerprints (banding patterns) corresponding to the sampled environment. In order to retrieve sequence data contained in these bands, which now constitute the partial 16S rRNA gene fragments of one single species, the bands were cut from the gel and placed in separate sterile vials holding 15 μl of highly purified sterile water. The DNA molecules diffuse out of the band in the water phase overnight (at 4◦ C).
FEMS Microbiol Ecol 71:428–443 van Doorn R, Klerks MM, van Gent-Pelzer MP, Speksnijder AG, Kowalchuk GA, Schoen CD (2009) Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel internal amplification control approach using Biotrove OpenArrays. Appl Environ Microbiol 75:7253–7260 Vrionis HA, Anderson RT, Ortiz-Bernad I et al (2005) Microbiological and geochemical heterogeneity in an in situ uranium bioremediation field site. Appl Environ Microbiol 71:6308–6318 Zhang G, Dong H, Xu Z, Zhao D, Zhang C (2005) Microbial diversity in ultra-high-pressure rocks and fluids from the Chinese Continental Scientific Drilling Project in China.