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By Badal C. Saha (Eds.)

Content material: 1. Commodity chemical compounds construction through Fermentation: an outline / Badal C. Saha -- 2. complicated non-stop Fermentation for Anaerobic Microorganism / Ayaaki Ishizaki -- three. Controlling Filamentous Fungal Morphology through Immobilization on a Rotating Fibrous Matrix to reinforce Oxygen move and L(+)-Lactic Acid construction by way of Rhizopus oryzae / Nuttha Thongchul and Shang-Tian Yang -- four. improving Butyric Acid construction with Mutants of Clostridium tyrobutyricum acquired from Metabolic Engineering and variation in a Fibrous-Bed Bioreactor / Ying Zhu and Shang-Tian Yang -- five. creation of Mannitol by way of Fermentation / Badal C. Saha -- 6. Fermentation technique improvement of Carbohydrate-Based Therapeutics / Chin-Hang Shu

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J. Fermentation Bioeng. 1992,74,379383. 23. T. Biotechnol. Bioeng. 2002,80, 1-12. 24. F. , pp. 457-507. 25. M. Biotechnol. Bioeng. 1998,57,409-419. 26. M. Biotechnol. Bioeng. 1998,60,2 16-229. 27. ; Okabe, M. J. Ferment. Bioeng. 1998,85,96-100. 28. -D. Bioprocess Eng. 1998, 19, 155-157. Chapter 4 Enhancing Butyric Acid Production with Mutants of Clostridium tyrobutyricum Obtained from Metabolic Engineering and Adaptation in a Fibrous-Bed Bioreactor Ying Zhu and Shang-Tian Yang* Department of Chemical Engineering, The Ohio State University, 140 West 1 9 Avenue, ~ ~ Columbus, OH 43210 Butyric acid has wide applications in food and pharmaceutical industries.

C*-CL) (2) where C* is the solubility of oxygen in the medium (mM)and kLa is the volumetric mass transfer coefficient (min-I). When there is no aeration, OTR = 0 and OUR (mM/min) can be determined fiom the slope of the plot of CL versus time during the period without aeration. Both kLa and C* can then be estimated fiom the plot of (dCL/dt+ OUR) versus CLduring the period with aeration, with kLaequal to the negative slope and C* equal to the Y-intercept divided by kLa. Time (min) Figure 3. A typical dissolved oxygen profile in the bioreactor obtained using the dynamic method of gassing out in the oxygen transfer experiment.

2000,26,87-107. B. Lactic acid. In: Moo-Young M, editor. Comprehensive Biotechnology. Oxford: Pergamon. 1985. pp. 761-776. -H. Appl. Biochem. Biotechnol. 1998,70-72,417-428. L. Process Biochem. 1999,34, 173-179. T. J. Biotechnol. 1995,41,59-70. Ho, W. Kinetics of L(+)-lactic acid production from glucose, xylose, and starch by free cells and immobilized cells of Rhizopus olyzae. MS Thesis, Ohio State University, Columbus, Ohio. 1996. ; Raimbault, M. World J. Microbiol. Biotechnol. 1994, 10,433-435.

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